BMIF is running several image analysis workshops offered free of charge to users of the BMIF. Workshop sessions are dependent on number of interests expressed.

ImageJ 101 (2-3 hours)
If you are new to digital image processing, or have little or no experience with ImageJ and would like an overview of some of the features commonly used by scientists, this workshop is for you.
We will explain terms such as pixels, bit-depth and look-up-tables, and walk you through the differences between destructive vs nondestructive operations. We will also aim to make it clear where enhancing an image ends and data falsification begins.
In the practical we will show you how to prepare images for publication and presentation, and touch on some entry level image analysis techniques.

Colocalisation workshop (2 hours)
This workshop is designed to give you a greater understanding of what it means for signals to ‘colocalise’ in microscopy images, and the different ways this can be interpreted. There are a range of colocalisation coefficients measuring different image parameters and claiming to report on colocalisation. We will endeavor to highlight the strengths and weaknesses of those most commonly cited.
The course will also cover how to correctly prepare and image your samples for colocalisation, and involve a hands on analysis using ImageJ.

If you are interested in learning about ImageJ 101 and/or colocalisation, please contact Michael Carnell, email: m.carnell@unsw.edu.au. Let him know your area of interest and if there are any other areas you would like covered in the future.

BMIF is also offering workshops discussing the use of MATLAB for image analysis. Workshops will focus on MATLAB Fundamentals including functions, scripts, plotting, and curve fitting. Other workshop topics will include Image Segmentation, Particle Tracking, Correlation Microscopy, and GUI Development.

Workshops are free of charge for active BMIF users.

If you are interested or have queries, please email Rusty Nicovich, email: p.nicovich@unsw.edu.au.

BMIF unites scientists dedicated to medical research, who address substantial scientific research questions of clinical relevance.

Falling within the UNSW Analytical Centre, the BMIF is a world-class imaging facility that enables innovative, cross-disciplinary research at the forefront of fundamental biomolecular sciences. The BMIF is particularly strong in cell and single molecule imaging.

The BMIF also leads Australia in the design, establishment and testing of new microscopes and imaging technologies. Our team work closely with international optic manufacturers to further advance technologies in bioengineering and bionanotechnology, which will aid our research towards finding answers to outstanding biomedical questions.

Currently the BMIF has twenty microscopes, available for research and analysis by students, academics and interested parties. The focus of the BMIF is fluorescence microscopy, examining samples illuminated by high energy light.

Our microscopes enable:

• Epifluorescence for live cell imaging
• Confocal Microscopy;
• Spinning Disc Microscopy
• Total Internal Reflection Fluorescence (TIRF) Microscopy
• Time-Resolved Single Molecule Imaging including Fluorescence Lifetime Imaging Microscopy (FLIM)
• Fluorescence Correlation Spectroscopy (FCS)
• 2-photon and Intravital Microscopy
• Stimulated Emission Depletion (STED) Microscopy

We also have other imaging modes such as atomic force microscopy (AFM).